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We used two types of stains on serial sections for each tissue. Using p63 immunohistochemistry, myoepithelial cell nuclei can be clearly detected, however intra-nuclear textures are not revealed. On the other hand, hematoxylin and eosin (HE) stained images require manual cell segmentation but reveal a wide spectrum of intra-nuclear textures. In addition HE stains also provide a basis for routine diagnostics by pathologists. We therefore performed comparative image analysis on both stains.
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