HMF was isolated from PSOs using a liquid-liquid extraction method and measured using an HPLC system (Agilent Infinity 1260, Agilent Technologies, Santa Clara, CA, USA) with a diode array detector (G1315D 1260 DAD VL, Agilent Technologies), quaternary gradient pump, and Agilent C18 column (250 × 4.6 mm, 5 µm, Agilent Technologies) according to the procedure reported in earlier works [35,36].
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