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2.6. Circular Dichroism (CD) Spectroscopy Determination

SZ Shunan Zhao
YD Yong Deng
TY Tianyi Yan
XY Xiaoling Yang
WX Weidong Xu
DL Donghong Liu
WW Wenjun Wang
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A J-1500 spectropolarimeter with a PM-539 detector (JASCO Inc., Tokyo, Japan) was utilized to determine CD spectra. The CD determination of zein protein solution without and with EA (different concentrations) in a cell of 1 mm light length was recorded from 190 nm to 260 nm at a scanning rate of 100 nm/min at 20 °C. The secondary structure of the protein was analyzed by using the dichroweb database.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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