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2.7. Intercellular Reactive Oxygen Species (ROS) Detection

GS Guoqing Sun
RZ Rui Zhang
CL Chengxiao Liu
WM Wenjun Meng
QP Qi Pang
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ROS level was assessed using a ROS kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) based on the manufacturer’s protocol. Briefly, SH-SY5Y cells were treated with DCFH-DA (5 μM) for 30 min in darkness. The fluorescence intensity of SH-SY5Y cells was determined with a FACScan flow cytometer.

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