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2.6. Staining Osteocyte Apoptosis by Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) Assay
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TUNEL staining was performed to detect osteocyte apoptosis (12156792910, Roche, Basel, Switzerland). The percentages of TUNEL-positive cells in osteocytes relative to 4′,6-diamidino-2-phenylindole (DAPI)-stained cells were calculated, and an analysis was conducted using a Leica immunofluorescence system (Leica Microsystems, Wetzlar, Germany). Photographs from three independent experiments were taken for each experimental group [25,32].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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