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T cell proliferation and cancer cell killing assays
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UV-irradiated MCA205-OVA as in ref. 59 were cocultured with BM-derived DCs (2:1 ratio, 24 h). DCs were cultured (5:1 ratio, 72 h) with splenic purified CD8+OT-1 cells. Cross-primed CD8+OT-1 cells were labeled with carboxyfluorescein succinimidyl ester (CFSE) dye (1 µM, 10 min, 37 °C) and restimulated with live PAR or CD44L MCA205-OVA cells (1:5 ratio, 3 days) before cytofluorometric CFSE level analysis on live-gated CD8+ cells and PI level analysis on CD45− cells.
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