2.6. Protein Array

Using the Cytokine Array #AAM-CYT-2000 (RayBio^® C-Series Mouse Cytokine Antibody Array C2000), we were able to detect 144 different cytokines in pancreatic tissue. We perfused the mice with PBS post mortem and removed the pancreatic tissue including the tumor. Mechanical lysis was performed prior to normalizing to the total amount of protein. The array was then tested according to the manufacturer’s instructions. The membranes were scanned and the signal intensity was quantified using ImageJ. Membranes with an irregular background were excluded from the analysis. The signal intensity of each dot was normalized to the positive control (=100). Duplicates of each cytokine were analyzed in eight KPPC mice and in six control mice (“control”).