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2.10. Enzyme-Linked Immunosorbent Assay (ELISA)
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After the CD8+ T cell-mediated cytotoxicity assay, the supernatants of each well were centrifuged at 300× g for 5 min and the cell debris was removed and used for ELISA. To measure cytokine levels, anti-mouse TNF-α ELISA kits (#MTA00B; R&D Systems, Minneapolis, MN, USA) were used. All processes were performed according to the manufacturer’s instructions.
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