Proteins that were judged significantly different between comparison groups were subjected to Gene Ontology (GO; https://www.blast2go.com/) analysis using Blast2 GO to annotate differentially expressed proteins that were classified by cell component, biological process and molecular function. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (http://www.genome.jp/kegg/) was used for pathway analysis of DEPs. The interaction network of DEPs was analyzed using the STRING database (http://string-db.org/). We selected the medium confidence (0.4) as the minimum required interaction score.
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