2.3.5. Contraction Activity of Extracts On an Isolated Wistar Rat Uterus Strip

Fresh uterine horns were prepared as described previously. After the first 10 minutes of negative control contractions, 1 ml of 0.5 mg/ml of the U. anisatum was introduced to the organ bath. Isometric contractions were recorded for 10 min followed by washing the strip three times with De Jalon's solution. A 30-minute uterine recovery time was allowed to normalize the contractions. Subsequently, the procedure was repeated with the 1.0 mg/ml, 2.0, and 4.0 mg/ml. The experiments were done in triplicates by employing unused strips in every dosage scale as well as rinsing the tissues well before each assay of the different extract dose [13]. The frequency and amplitude of uterine contractions was calculated and recorded.