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The validation of reproducibility
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Intra-assay reproducibility tests were carried out in triplicate by respectively testing three different concentration mixtures (105 and 103 copies/ml and a lowest limit of detection, either 500 or 250 copies/ml) of each pathogenic nucleic acid within the same experiment. The inter-assay variability was examined by repeating the intra-assay run on three continuous days to validate the reproducibility of the multiplex real-time RT-PCR.
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