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Bacterial load determination.

KS Kristin Scoggin
JG Jyotsana Gupta
RL Rachel Lynch
AN Aravindh Nagarajan
MA Manuchehr Aminian
AP Amy Peterson
LA L. Garry Adams
MK Michael Kirby
DT David W. Threadgill
HA Helene L. Andrews-Polymenis
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Mice were humanely euthanized by CO2 asphyxiation, and the spleen, liver, ileum, cecum, colon, Peyer’s patches, and MLN were collected. A third of each organ was collected in 3 mL of ice-cold phosphate-buffered saline (PBS), weighed, homogenized, serially diluted in 1× PBS, and plated on Nal plates for enumeration of S. Typhimurium in each organ. Peyer’s patches and MLN were collected whole. The data are expressed as CFU/g of tissue.

Copyright and License information:  ©2022 Scoggin et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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