Extracted DNA were fractionated by density gradient ultracentrifugation using cesium chloride (CsCl) as previously described [12]. Centrifuged fractions of approximately 340 μL were collected from top to bottom using 1 mL syringes fitted with 21-gauge needles. For each fraction, the density was measured using a 3225 refractometer (Carl Zeiss, Oberkochen, Germany), and DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and purified from CsCl salts with the Geneclean Turbo Kit (MP Biomedicals, Santa Ana, USA). For each gradient, two fraction representatives of labeled DNA (heavy-DNA fractions) and two representatives of unlabeled DNA (light-DNA fractions) were selected.
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