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Placement of catheter for imaging

SM Signe H. Mikkelsen
BW Boris Wied
VD Vitalii Dashkovskyi
TL Thomas Beck Lindhardt
LH Lydiane Hirschler
JW Jan M. Warnking
EB Emmanuel L. Barbier
DP Dmitry Postnov
BH Brian Hansen
EG Eugenio Gutiérrez-Jiménez
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For administration of dyes and drugs during imaging, a venous catheter can be placed in the tail of the mouse. For this procedure, the mouse was anesthetized (induced with 3% Isoflurane in 100% oxygen and maintained with 1.5–2% Isoflurane in 100% oxygen using a mask) to place the catheter. The catheter was fixed with dental acrylic before injection. It is recommended to warm up any administered fluid to body temperature to avoid pain during injection. The mouse was positioned in the optical imaging bed during anesthesia waning and regained consciousness while restrained.

Copyright and License information:  ©2022 Mikkelsen, Wied, Dashkovskyi, Lindhardt, Hirschler, Warnking, Barbier, Postnov, Hansen and Gutiérrez-Jiménez.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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