Forced swim test

The forced swim test (FST) was conducted in a plexiglass cylinder with a diameter of 18 cm and a height of 27 cm. The water depth was 15 cm, and the water temperature was kept at 25 ± 1°C. When testing, mouse cannot be supported by their limbs or tail. Baffles were placed between adjacent cylinders to prevent mice from learning from each other. The water in the cylinder was replaced every three mice to avoid the interference of excreta. The forced swim test was divided into two stages:1) Adaptation stage: 1 day before the test, mice were forced to swim for 15 min. 2) Forced swim test: 24 h later, mice were subjected to the swimming test for 6 min, and the floating immobility time of the last 4 min after recording was used as an indicator of depressive-like and despair behavior (Snyder et al., 2011).