Novelty suppressed feeding test

The novelty suppressed feeding test (NSFT) was performed in an opaque acrylic plate box with an open top (42 cm × 42 cm × 42 cm). Mice were tested after 24 h of food deprivation. Four food pellets from home cage were placed in the central area of the test box. Mice were gently put into the test box from one corner. The latency to start eating food pellets was used to evaluate the anxiety-like behaviors. During the test, mouse was allowed to explore freely in the box for 10 min, and the longest latency was recorded as 600 s (Linge et al., 2013).