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Genomic DNA extraction and chop-PCR analysis

YW Yaqin Wang
HL Hui Liu
ZW Zhanqi Wang
YG Yushuang Guo
TH Tao Hu
XZ Xueping Zhou
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Total genomic DNA was isolated from leaf samples using a Super Plant Genomic DNA Kit (Tiangen, Beijing, China) according to the manufacturer’s instructions. Approximately 300°ng genomic DNA was digested with a methylation-sensitive restriction enzyme HpaII, as described previously (Li et al., 2020). Chop-PCR was carried out as described previously (Zhong et al., 2017a; Kong et al., 2020).

Copyright and License information:  ©2022 Wang, Liu, Wang, Guo, Hu and Zhou.
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