CDAA-HFAT and Western Diet to Induce NASH Fibrosis

A CDAA-HFAT (Research Diets, New Brunswick, NJ) or choline-supplemented, L-amino acid-defined control diet was fed to mice for 10 weeks starting at the age of 8 weeks. As a second dietary NASH model, a Western diet (high fat, fructose and cholesterol diet and water supplemented with sucrose/fructose) (AIN-76A Western Diet, TestDiet, St. Louis, MO) or chow control diet was fed to mice for 20 weeks starting at the age of 8 weeks. For dietary experiments, groups (knock-outs and controls) were cohoused. Mice fed CDAA diet show severe NASH characterized by steatohepatitis and fibrosis.