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2.2. Virus Strains and Viral Samples
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The inactivated antigens of classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine pseudorabies virus (PRV) and porcine epidemic diarrhea virus (PEDV) were kept in our laboratory. Viral DNA and RNA were extracted using the QIAmp Viral DNA/RNA kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. Viral RNA was reverse transcribed to cDNA using Primescript II 1st strand cDNA Synthesis kit (TaKaRa, Dalian, China). All of the DNAs and cDNAs templates were stored at −80 °C until use.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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