The XRD analysis was carried out to observe the CS/ESP/OPAC film’s possible crystalline structure. The crystal structures of the CS/ESP/OPAC film were studied using diffraction patterns acquired on an X-ray diffractometer Rigaku Smartlab XRD/6000 (Austin, TX, USA). The crystallinity index (CI) of CS/ESP/OPAC film was calculated based on the calculus of crystallinity area (Ac) and amorphous area (Aa) in a diffractogram using the following equation [36].
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