2.12.4. Trolox Antioxidant Assay (ABTS)

A modified ABTS test was used to determine the antioxidant capability of the biosynthesized NPs [38]. In equal portions, potassium per sulphate (2.45 mM) and 7 mM ABTS salt were mixed and incubated at room temperature overnight. After incubation, samples were put in the mixture and kept at room temperature in the dark for 15 min. The BioTek ELX800 was used to measure the absorbance of the sample in the reaction mixture at a wavelength of 734 nm. The Trolox reagent was utilized as a positive control in this test, whereas DMSO was employed as a negative control. In a triplet experiment, the findings (antioxidant potential) were represented as TEAC.