4.8. Statistical Analysis

The mean and standard error were used to express all of the findings (SEM). The data were analyzed using a two-way ANOVA followed by a Bonferroni post hoc test and a one-way ANOVA followed by a Tukey’s multi-comparison test. It was determined that p < 0.001 was statistically significant. The sample size was estimated after the data was confirmed to be normalized, and the normality distribution was checked using the Kolmogorov Smirnov test. GraphPad Prism version 5.03 for Windows generated all statistical results (GraphPad Software, San Diego, CA, USA). The mean and standard error of the mean was used to express the statistical data (SEM).