Transwell migration and wound healing assay

Wounds were made with pipette tips on A549 or H1299 cell monolayers, and the cells were cultivated for 48 hours at 37 °C with 5% CO₂. Sections of the wounds were imaged before and after incubation. The degree of wound repair was calculated using ImageJ (NIH, USA). Migration assays were performed using a transwell with a pore size of 8.0 um (Millipore), according to the manufacturer's instructions.