Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Measurements of intracellular Na+ and K+ concentrations
Request a Protocol
Ask a
question
Favorite
For intracellular Na+ ([Na+]i) and K+ ([K+]i) measurements, cells were grown in 10 cm tissue culture plates and treated with the various osmotic solutions (Table S2) for 15 min. They were then quickly washed 3 times with osmotically balanced solution of 150 mM LiCl (300 mOsm) on ice. The cells were then lysed overnight at 4 °C in 1% HNO3. The [Na+]i and [K+]i were measured using a PinAAcle 900F Atomic Absorption Spectrometer (PerkinElmer) and then normalized to cell number and cell volume.
Copyright and License information: The Author(s) ©2022
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
