2.9. Glucose Consumption and Lactate Production

Colorimetric assay was used for the assessment of glucose and lactate concentration in cell culture medium. After a 48 h transfection, 1 mL supernatant was gently aspirated into a new Eppendorf (EP) tube. Fresh complete medium (1 mL) was aspirated into a centrifuge tube as a blank group. The sample was centrifuged at 2500 rpm for 10 min, and the supernatant was taken for determination. The reagents and samples were added step by step following the instructions of the kit (KA4086, Abnova, Walnut, CA, USA), mixed, and water-bathed at 37°C for 15 min, and the OD values of each well were assessed. The lactate production was measured in the same way (BC2230, Solarbio) except for no centrifugation.