4.5. Serum Triglyceride Measurement

Serum TG (mg/L) were measured using the Cayman kit (cat. no. 10010303). Briefly, 10 µL of serum sample was added to 2 wells in a 96-wells polystyrene microplate and mixed with 150 µL of enzyme solution (ingredients were supplied with the kit) with multichannel pipette. The plate was incubated for 15 min at room temperature. Absorbances for both sample and standard were measured at 540 nm.