2.3.1. Growth and Sporulation

Five laboratory fludioxonil-resistant mutants (TY-1-fR, TY-3-fR, TY-5-fR, TY-8-fR, and TG-5-fR) and their parental isolates (TY-1, TY-3, TY-5, TY-8, and TG-5) were used. The mycelial growth was compared using the protocols of previous studies [18]. Briefly, mycelial plugs (5 mm) were taken from the edge of 3-day-old colonies and transferred to fresh PDA. The dishes were incubated at 24 °C, and the resulting colonies observed daily; the diameter of each was measured at 24, 48, 72, 96, and 120 h post inoculation (hpi). Each isolate was represented by five separate dishes, and the entire experiment performed in triplicate. Meanwhile, the sporulation of the five mutants and their parental isolates was assessed as macroconidia produced using the method detailed in a previous study [18], with modifications. Briefly, mycelial plugs (5 mm) were taken from the edge of 3-day-old colonies and transferred to fresh PDB medium, after incubation at 24 °C with shaking (120 rpm) for 4 days, the resulting macroconidia were harvested and counted using a hemocytometer (Shanghai Qiujing Biochemical Reagent Instrument Co., Ltd., Shanghai, China) after filtration with a double-wrapped sterile gauze. Each isolate was represented by five separate flasks, and the entire experiment performed twice. Fisher’s least significant difference test in the SPSS software (ver. 17.0; SPSS Inc., Chicago, IL, USA) was calculated to determine statistical significance among different mutants (or isolates).