Determination of strength properties of tissue adhesive

Porcine skin was cut to dimensions (Fig 7) and kept moist at all times with PBS. The backside of the tissue sample was glued to the test fixture using cyanoacrylate glue. Before testing, the test fixture was equilibrated to the temperature of 37±1°C.

Tissue adhesives were prepared according to the manufacturers’ instructions. A sufficient amount of adhesive was applied to the test fixture to coat the over-lap area uniformly without significant overflow. The two sides of the test fixture were bonded together and incubated at room temperature for 5 min.

The test specimens were wrapped with gauze soaked in PBS and were placed in an environmental chamber at 37°C for 1 hour. Before testing, the test specimens were stabilized for 15 minutes at room temperature.

The test specimens were placed in tensile machine grips (Fig 8) and tested according to ASTM F2255-5 [27].