MDA-MB-231, SUM159, and 4T1 cells were seeded in a 6-well plate and cultured until fully confluent. A straight scratch crossing the monolayer was created using a 200 µL pipette tip and images of the scratched areas were taken. Following this, the cells were treated with DMSO or LLL12B and images were taken until the scratch of the DMSO-treated cells was closed and the relative migration (%) was calculated.
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