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PBMC from 30 IRIS and 30 non-IRIS samples were isolated using standard Ficoll-histopaque (GE Healthcare Bio-Sciences AB) density gradient centrifugation. PBMC were stored at − 80 °C in cryoprotective medium (RPMI-1640 [Sigma-Aldrich], 10% fetal calf serum [FCS; Cell Technology Inc], 10% dimethylsulfoxide [DMSO; Sigma-Aldrich]). RPMI containing 10% FCS is hereafter referred to as R-10.
Copyright and License information: ©2017 The Francis Crick Institute
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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