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Liver tissue: gene mRNA expression analysis

RB Ritesh K. Baboota
AR Aidin Rawshani
LB Laurianne Bonnet
XL Xiangyu Li
HY Hong Yang
AM Adil Mardinoglu
TT Tamar Tchkonia
JK James L. Kirkland
AH Anne Hoffmann
AD Arne Dietrich
JB Jeremie Boucher
MB Matthias Blüher
US Ulf Smith
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RNA from human liver biopsy donors was extracted using RNeasy Lipid Tissue Mini Kits (Qiagen). The quantity and integrity of the RNA were monitored with a NanoVue Plus Spectrophotometer (GE Healthcare). A total of 1 µg of RNA was reverse-transcribed with standard reagents (Life Technologies). Complementary DNA was then processed for TaqMan probe-based quantitative real-time PCR (RT–qPCR) using a QuantStudio 6 Flex Real-Time PCR System (Life Technologies). The expression of target genes was calculated by the standard curve method and normalized to the expression of hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) as a housekeeping gene. The primers and probes used are listed in Supplementary Table 5.

Copyright and License information: This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply ©2022
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