Quantification of enzyme activities

YS Yang Sun
WG Wenwen Guo
FW Fen Wang
CZ Chunjun Zhan
YY Yankun Yang
XL Xiuxia Liu
ZB Zhonghu Bai
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Quantification of the pyruvate carboxylase (PC), isocitrate dehydrogenase (ICDH), glutamate dehydrogenase (GDH) activities was performed according to modified protocols. The protein concentration was quantified using the Bradford assay. Specific activities were calculated as follows: as = a/TP [17].

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