2.1. Cell Culture and Grouping

Human GC SGC7901 lineage cell SGC7901 was acquired from Shanghai Cell Bank, Chinese Academy of Sciences. The human GC SGC7901 lineage cell was cultured with 10% FBS (Gibco, America) and {"type":"entrez-protein","attrs":{"text":"RPM11640","term_id":"1520783453"}}RPM11640 medium (Gino Biopharmaceutical Co., Ltd.) with 1% double-antibody placed in an incubating device under 37°C and 5% CO2. Follow-up experiments are carried out when the cell confluence reaches 70-80%. The concentration of IL-8 (Boaosen Biotechnology Co., Ltd.) is screened by the MTT method, the old medium of human gastric cancer SGC7901 cell line is discarded, and an equal volume of PBS, 20 ng/mL, 40 ng/mL, and 60 ng/mL IL-8 new medium, respectively, control group, IL-8 (20 ng/mL) group, IL-8 (40 ng/mL) group, and IL-8 (60 ng/mL) group, and continued to incubate for 24 h. In order to verify the effects of the PI3K/Akt signal path on the modulation of autophagy in GC SGC7901 cells, they were separated into the control group, IL-8 group, LY294002 group, and IL-8 + LY294002 group; the control group was added the same volume of PBS to the mankind GC SGC7901 lineage cell; the IL-8 group was added with IL-8 at a concentration of 60 ng/mL in the mankind GC SGC7901 lineage cell; the LY294002 group was added with LY294002 at a content of 20 μmol/L (purchased from APEXBIO, USA). IL-8 at a content of 60 ng/mL and LY294002 at 20 μmol/L were supplemented into the IL-8 + LY294002 group.