In vivo leukemia analysis: Subcutaneous model

HL-60 Cells (5 × 106) were implanted subcutaneously in a Matrigel matrix (1:1) onto the flank of each mouse and allowed to grow to the pre-specified size of 150 mm3. The mice were not pre-treated with an agent prior to their implant. Mice were grouped randomly into treatment groups based on their bodyweight to ensure even distribution. Mice were treated as indicated in Table 2. Tumors were measured 3 times per week using digital calipers. An independent scientist was assigned to measuring the tumors in the study and was blinded from knowing which treatment group the animal was in. Tumor volume measurements were taken three times weekly and the only information the measuring scientist would see is the cage number and the animal’s identification mark. The length and width of the tumor were measured, and volume calculated using the following formula: volume = (length x width²)/2. The tumor control ratio (T/C) was calculated in the following way: ((Mean tumor volume on day 28 – mean starting volume)/(Mean vehicle tumor volume on day 28 – mean vehicle starting volume))∗100. The study was terminated the end of the 28-day treatment period.