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Thorase homozygous KO mice and wild type littermates were obtained by crossing heterozygous Thorase mice14,16. Animal experiments were performed in compliance with the regulations of the Animal Ethical Committee of the Johns Hopkins University Animal Care and Use Committee. Wild type or Thorase KO mice were injected intraperitoneally with 6mk/kg Rapamycin (#R-5000, LC laboratories) or vehicle (10% PEG400, 10% Tween 80 in water), three times per week starting at 10–14 days postnatally.
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