Cell culture and hawthorn fruit acid treatment

After the cells grew to 80% confluence, the medium was changed and drugs were added. The experiments were divided into five groups. The groups were as follows: (i) control group; (ii) OA treated group; 0.1 mM OA incubated for 18 h; (iii) OA + simvastatin group; 100 μM OA and 5 μM simvastatin incubated for 18 h; (iv) OA + HFA high dose group; 100 μM OA and 0.25 mg/mL HFA incubated for 18 h; and (v) OA + HFA low dose group; 100 μM OA and 0.125 mg/mL HFA incubated for 18 h.