Role of NaSa in Staphylococcus aureus ATCC 25923 growth kinetics and viability

The effect of NaSa on the growth kinetics of S. aureus ATCC 25923 was evaluated at a cell density of 10⁷ CFU/ml in TSB, with and without 50% fetal bovine serum (HyClone Laboratories, Logan, United States), and supplemented with 0.1, 1 or 10 mM NaSa. TSB alone (media control), the inhibition controls: TSB supplemented with 10% glucose (Merck, Darmstadt, Germany), 1 μM AIP-I and 10% AIP-II containing supernatant, as well as the agr activator control AIP-III containing supernatant were included. Two hundred microliters of each sample in duplicate was added to 96-well microtiter plates (Thermo Fisher Scientific, Waltham, United States), and the OD at 600 nm was measured at 37°C every 30 min for 24 h using a microplate reader. After the final read, 100 μL were taken to perform CFU counting as described above.