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RAW264.7 cells were transfected with a previously generated19 V5-tagged wild-type CRELD2 cDNA construct in pcDNA3.1 (+) to overexpress CRELD2 and an empty pcDNA3.1 (+) plasmid as a control. Co-immunoprecipitation was performed using anti-V5-agarose affinity gel (Abcam) to pull out V5-tagged CRELD2 and the resulting interacting partners as outlined previously19. CRELD2 interacting proteins were then identified by western blotting.
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