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Annexin V-FITC/propidium iodide (PI) apoptosis assay
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Annexin V-FITC and PI were detected by flow cytometry using two channels to determine the proportion of apoptotic cells. The drug-treated suspension and adherent cells were collected into a flow tube and incubated with 5 μL Annexin V-FITC and 10 μL PI in a dark room at room temperature for 15 min. Data were obtained using Beckman Cytoflex LX flow cytometry (Beckman Coulter, USA) and processed using CytExpert software (Beckman Coulter, USA). Each experiment was repeated three times independently, and the data were statistically analyzed.
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