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The splenocyte IFN-γ secretion levels were quantified by using the Mouse IFN-γ ELISPOT Set (BD Biosciences, USA) following the manufacturer's protocol. The cells were stimulated by 24 h incubation with OVA peptide 257-264 (SIINFEKL, GenScript, China) before secondary antibody treatment. IFN-γ spot-forming cells were counted by an ImmunoSpot Analyzer ELISPOT reader (Cellular Technologies Ltd, USA).
Copyright and License information: ©2022 Zheng Gong et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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