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Human tumor-infiltrating lymphocyte flow cytometry
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Dissociated tumor biopsies were obtained from Conversant Bio with informed written consent and according to Institutional Review Board (IRB)-approved guidelines in accordance with the Declaration of Helsinki. Subject information is detailed in Supplementary Table S2. Tumor samples were thawed and washed with PBS, blocked with Human Fc Block (BD Biosciences) before staining with fluorophore-conjugated antibodies for 30 minutes at 4°C. Cells were washed and resuspended in PBS for flow cytometric analysis.
Copyright and License information: ©2022 The Authors; Published by the American Association for Cancer Research
This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.
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