Adult male Wistar rats (380–400 g; ~ 3-month-old at arrival; Janvier) were housed in groups of four per large polycarbonate cage (80 × 60 × 60 cm), and housed under standard climate-controlled housing conditions with a 12 h light cycle and free access to water and rat chow. After one week of acclimation, rats were habituated and randomly allocated to two groups. All parts of the in vivo study, including the stereotactic injections, behavioral tests, MRI scanning and the following histological analyses, were performed by investigators who were blinded to the treatment. Injections and all behavioral experiments were performed with 12 rats per group. At the end of the in vivo study, 6 rats from each group were randomly selected for MRI analysis, whereas another 6 rats from each group were used for ex vivo analysis.
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