Animals

EB Eva Baerends
KS Katia Soud
JF Jonas Folke
AP Anna-Kathrine Pedersen
SH Simon Henmar
LK Lisa Konrad
ML Matthew D. Lycas
YM Yuki Mori
BP Bente Pakkenberg
DW David P. D. Woldbye
OD Oksana Dmytriyeva
SP Stanislava Pankratova
request Request a Protocol
ask Ask a question
Favorite

Adult male Wistar rats (380–400 g; ~ 3-month-old at arrival; Janvier) were housed in groups of four per large polycarbonate cage (80 × 60 × 60 cm), and housed under standard climate-controlled housing conditions with a 12 h light cycle and free access to water and rat chow. After one week of acclimation, rats were habituated and randomly allocated to two groups. All parts of the in vivo study, including the stereotactic injections, behavioral tests, MRI scanning and the following histological analyses, were performed by investigators who were blinded to the treatment. Injections and all behavioral experiments were performed with 12 rats per group. At the end of the in vivo study, 6 rats from each group were randomly selected for MRI analysis, whereas another 6 rats from each group were used for ex vivo analysis.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A