Xanthomonas oryzae pv. oryzae Resistance

Xanthomonas oryzae pv. oryze strain PXO99A was used to infect rice plants (Salzberg et al., 2008). X. oryzae pv. oryzae was grown on peptone sucrose agar plates for 3 days and resuspended in water for an OD₆₀₀ of ∼0.5. Five-week old greenhouse grown plants were transferred to controlled growth chambers set to 28°C, 80% relative humidity, and 14 h light and 10 h dark cycling. Plants were left to acclimate for 2–3 days before inoculations. Plants were inoculated by cutting leaf tips with scissors dipped in X. oryzae pv. oryzae suspension media (Kauffman et al., 1973). Lesion measurements were scored 14 days after scissor inoculation.