EXPERIMENTAL MODEL AND SUBJECT DETAILS

Parental (wildtype) HeLa cells (Human cervix carcinoma, female) were purchased from ATCC (Cat. #: CCL-2). HeLa cells with CRISPR-Cas9 mediated ablation of NAT10 were previously generated and validated (Arango et al., 2018). Parental Flp-In 293T-REx (Human embryonic kidney, female) were purchased from ThermoFisher Scientific (Cat. #: {"type":"entrez-nucleotide","attrs":{"text":"R71007","term_id":"844524","term_text":"R71007"}}R71007). Generation and validation of 293T-Rex cells stably overexpressing NAT10 was previously reported (Arango et al., 2018). Wildtype HeLa, NAT10−/− HeLa, Flip-In 293T-REx, and 293T-REx cells overexpressing NAT10 were cultured in Dulbeccos’ Modified Eagle Medium (DMEM, ThermoFisher Scientific, Cat. #: 10313021) containing 25 mM glucose, 1 mM sodium pyruvate and supplemented with 2 mM L-glutamine (ThermoFisher Scientific, Cat. #: 25030149) and 10% bovine calf serum (BCS, HyClone, Cat. #: SH30073.03), in the absence of antibiotics.