Materials

The following reagents were purchased from commercial sources: Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) from HyClone (Logan, UT, USA); trypsin and ethylenediamine tetraacetic acid (EDTA) from Amresco (Solon, OH, USA). Mouse anti-human Lewis(y) monoclonal antibody (clone A70-C/C8; ab217909) was purchased from Abcam (Cambridge, UK). Rabbit anti-human MUC1 polyclonal antibody (sc-15333), HRP-labeled second antibodies (sc-51948) and protein G plus-agarose (sc-500778) were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Goat monoclonal anti-mouse immunoglobulin E tetramethylrhodamine isothiocyanate (TRITC; ZF-0313) and goat monoclonal anti-rabbit immunoglobulin G fluorescein isothiocyanate (FITC; ZF-0311) were purchased from Zhongshan Biotechnology (Beijing, China). The immunohistochemical SP kit was purchased from Mai Xin Co. (Fujian, China). TRIzol reagent, the PrimeScript™ RT reagent kit and SYBR^® Premix Ex Taq™ were purchased from Takara Biotechnology Co. (Dalian, China). The sequences of the primers were synthesized by Invitrogen Co. (Shanghai, China).