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IHC was performed on pancreatic tissue as previously described [22]. Slides were incubated overnight with primary antibody (Supplementary Table 1) and developed with Nova Red (Vector Labs) and hematoxylin counterstain. Five random fields (40× NA 0.65 A-Plan objective using Mikroscan D2 Digital Slide Scanner with Q-Skan) per pancreas were imaged and counted [23]. Each field contained at least 1000 cells.
Copyright and License information: ©2017
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