2.3. Biofilm Formation Assays

Strains were grown in LB to an optical density at 600 nm (OD₆₀₀) of 1.0. For the colony assay, the LB cultures were 10 times diluted with sterile deionized water and spotted on the MSgg agar (1.5% agar), and the seeded agar plates were incubated at 30 °C for different times. For the pellicle assay, 1.5 μL of the diluted culture was added to 1.5 mL LBMG medium contained within a well of a 24-well microtiter plate, and the microtiter plates were incubated at 30 °C for the indicated times.