3.6.3. Experimental Design

There were seven rat groups, each with six rats. The test samples were administered to all the rats once daily for 21 days. Additionally, 0.1% CMC (Carboxymethylcellulose) in distilled water was used as a suspending agent. As per the literature survey, the Psidium guajava extract was found to be safe for up to 5000 mg/kg body weight [27].

Group I: Normal control (CMC 0.1%);

Group II: Diabetic control received STZ (45 mg/kg b.w.i.p.);

Group III: Rats with diabetes treated with Metformin (100 mg/kg b.w.p.o.);

Group IV: Rats with diabetes treated with Psidium guajava extract (200 mg/kgb.w., p.o);

Group V: Rats with diabetes treated with Psidium guajava extract (400 mg/kgb.w., p.o);

Group VI: Rats with diabetes treated with Psidium guajava nanoparticles (200 mg/kgb.w., p.o);

Group VII: Rats with diabetes treated with Psidium guajava nanoparticles (400 mg/kgb.w., p.o).