2.3.2. Ferric Reducing Antioxidant Power (FRAP) Assay

FRAP assay was conducted in triplicates (n = 3), where 175 µL of freshly prepared FRAP reagent (10 mM TPTZ (2,4,6-tripyridyl-S-triazine) in 40 mM in HCl (10 Mm), acetate buffer (300 mM, pH 3.6), 20 mM FeCl₃) were mixed in a 96-well microplate with 25 μL of mango methanol extract (prepared in Section 2.2.1). The mixture was then incubated in the dark for 30 min and recorded at 593 nm afterward using the Gen 5 UV/Vis microplate reader (BioTek Instruments, Inc., Winooski, VT, USA). A Trolox and ascorbic acid (AA) solution was used to construct the assay’s calibration curves in a concentration curve of 0.01–0.1 mg/mL. The results (mean ± SD) were expressed as mg Trolox and AA equivalents per g extract as mg TE/g extract and mg AA/g extract, respectively [25].

Statistical analysis using one-way ANOVA with the aid of the Tukey method (p < 0.05) was applied for the classification and grouping of investigated mango cvs. based on TPC and antioxidant effect.