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Taxonomic Assessment of Frankliniella occidentalis Microbiota From Existing Sequence Data

AA Awawing A. Andongma
MW Miranda M. A. Whitten
RS Ricardo Del Sol
MH Matthew Hitchings
PD Paul J. Dyson
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A custom Kraken2 database (Wood et al., 2019) was constructed to encompass the following NCBI RefSeq categories: bacteria, archaea, fungi, plant, protozoa, viral and plasmid using the Kraken2-build process. Additionally, the genomes of BFo1 (GenBank Accession: GCA_001602545.), BFo2 (GenBank Accession: GCA_001602605.1) and F. occidentalis (GenBank Accession: GCA_000697945.4) were also added to the library with existing NCBI taxonomy IDs used: NCBI:txid1628855, NCBI:txid1628856 and NCBI:txid133901, respectively. Short paired-end sequence reads from the reference F. occidentalis hologenome sequence, BioProject accession PRJNA203209, (285,047,552 in total) were queried against the custom Kraken2 database. Graphical representation of read proportions were generated using the R packages cowplot, ggforce and tidyverse.

Copyright and License information:  ©2022 Andongma, Whitten, Sol, Hitchings and Dyson.
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